Coding

Part:BBa_K4361311

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-19)
Revision as of 13:05, 19 September 2022 by Registry (Talk | contribs)

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BlcR L66A

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.2 L66V (Part:BBa_K4361220). For this mutant, the leucine in position 66 has been changed to alanine by mutating the CTC codon to GCG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589


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