Coding

Part:BBa_K4361305

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-19)
Revision as of 12:37, 19 September 2022 by Registry (Talk | contribs)

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BlcR A62I

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 (Part:BBa_K4361210) and F7.3 A62I (Part:BBa_K4361213). For this mutant, the alanine in position 62 has been changed to isoleucine by mutating the GCG codon to ATT.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589


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