Composite

Part:BBa_K4286099

Designed by: Minxi Zeng，Yingfeng Wu   Group: iGEM22_SZU-China   (2022-09-19)
Revision as of 08:34, 19 September 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Classical oscillator composed of three genes encoding repressor protein

2022 SZU-China has designed an auto-timing kill switch mediated by a classical oscillator. In the negative feedback loop of the oscillator, the oscillator is composed of three genes encoding repressor protein -- TetR from the Tn10 transposon, Ci from bacteriophage lambda, and LacI from the lactose operon -- each repressor protein is attached to an LVA rapid degradation tag located at the carboxy terminus. The concentration of each kind of repressor protein changed cyclically: lacI inhibited the expression of tetR, tetR inhibited the expression of cI, and cI inhibited lacI expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None