Part:BBa_K4179001
Pastinaca sativa prenyl transferase 1 (PsPT1)
P. sativa prenyltransferase 1 (PsPT1) was discovered and characterized as a plastidic prenyltransferase, with 6 predicted transmembrane helices, that synthesize demethylsuberosin (DMS), a linear furanocoumarin (FC) intermediate, and osthenol an angular FC intermediate, respectively, in parsnip. Prenylation is a structural modification in which an isoprenoid moiety, prenyl being the most common, is transferred from a donor molecule to an acceptor. Prenylation of aromatic substrates is known to enhance their bioactivity and is an essential step in the biosynthesis of biologically active secondary metabolites, which play an integral role in the survival and disease resistance of many plant species. To perform prenylation, plants use membrane-bound aromatic prenyltransferases (PTs) that transfer isoprenoid moieties from pyrophosphate donor substrates to aromatic acceptor substrates. PsPT1 is an umbelliferone dimethylallyltransferase (UDT), a prenyltransferase that is specific for the first reaction step in the FC biosynthetic pathway. It mediates a prenylation reaction in which umbelliferone is the acceptor of the prenyl group, and dimethylallylpyrophosphate (DMAPP) is the donor, producing DMS of the prenylation took place at the C6 position of umbelliferone, and osthenol if prenylation occurred in the C8 position. Crude leaf microsomes were prepared by Munakata et al., to investigate angular FC synthesis in parsnips. Incubation of the microsomes with umbelliferone, DMAPP, and the cofactors required for the reaction generated DMS and, to a lesser extent, osthenol, thus indicating that the UDTs in parsnip favor the formation of DMS over osthenol.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal PstI site found at 55 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal NheI site found at 1210
Illegal PstI site found at 55 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal PstI site found at 55 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal PstI site found at 55 - 1000COMPATIBLE WITH RFC[1000]
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