Translational_Unit

Part:BBa_K4158006:Design

Designed by: Izumi Kim   Group: iGEM22_Waseda_Tokyo   (2022-09-18)
Revision as of 06:47, 18 September 2022 by S Okuda (Talk | contribs) (→‎Design Notes)


RBS-AtzB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to get same plasmid that we experimented with, construct the plasmid along this way below.

1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick.

2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI.

Source

test

References