Coding

Part:BBa_K4348008

Designed by: Rylan Donohoe   Group: iGEM22_McGill   (2022-09-17)
Revision as of 17:07, 17 September 2022 by Registry (Talk | contribs)

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AKR1D1_E28toGap_his

This is the gene for the human AKR1D1 enzyme with a mutation on the E28 residue, turning it into a gap. The mutation was done so it may have more activity on 4-cholesten-3-one, which is not its wild-type ligand.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None