Composite

Part:BBa_K4122014

Designed by: JIAHUA FANG   Group: iGEM22_IvyMaker-China   (2022-09-15)
Revision as of 07:16, 15 September 2022 by Registry (Talk | contribs)

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PFBA1-SP-GFP-His-Spytag-TADH2

In this assembly, GFP is a green fluorescent protein, showing green fluorescence, His-tag is used for protein purification, and Spytag will form a tight connection with spycatcher, which in turn anchors the attached GFP to the cell surface.Promoter-PFBA1, Terminator-TADH2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None