Primer

Part:BBa_K4308000

Designed by: Jun Zhang   Group: iGEM22_CPU_CHINA   (2022-09-14)
Revision as of 13:58, 14 September 2022 by ZHANG JUN (Talk | contribs)


Forward Primer for RPA of LINC00857

Long intergenic non-protein coding RNA 857 (LINC00857) is a genetic molecule involved in cancer biology, which is identified as one of the most upregulated LINCRNAs in lung cancer. It could lead to resistance to platinum‐induced apoptosis by affecting mRNA stability or promoting its translation. Thus, we chose LINC00857 as a novel prognostic and cancer predictive biomarker. We use this part as the forward primer to conduct recombinase-aid amplification (RAA) of LINC00857, along with its reverse primer (BBa_K4308001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None