Coding

Part:BBa_K190018:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-07-15)
Revision as of 10:01, 3 August 2009 by Jelle (Talk | contribs) (→‎References)

HmtA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 646
    Illegal XhoI site found at 1009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 559
    Illegal NgoMIV site found at 1286
    Illegal NgoMIV site found at 1460
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1763
    Illegal NgoMIV site found at 1772
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Still contains a EcoRI site at position 6.

Two PstI sites have been removed

Source

gi|81857196|sp|Q9I147|Q9I147_PSEAE

Derived from construct used for paper:

Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-4682

References

Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-468