Part:BBa_K4235003

Gentamicin Resistance Gene

Usage and Biology

Introduction: Gentamicin is an aminoglycoside frequently used in clinical settings to treat many bacterial infections. A gentamicin resistance gene can confer resistance to aminoglycosides based on several mechanisms: enzymatic inactivation of aminoglycosides, increased efflux from the bacterial cell, interfering with binding domains of aminoglycosides, etc.

Usage and Biology:

This part is the entire circuit of the Gentamicin Resistance gene which is used within the plasmid (pFastBac-YmBac2) and it confers resistance to gentamicin. The expression of the gentamicin coding sequence is driven by the pC promoter (add link). This circuit is also a part of the mini-attTn7 transposon segment (add link), used in the Bac-to-Bac expression system by Stony_Brook 2021.

This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 314
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

This Gentamicin resistance gene is part of the original plasmid pFastBac, which was a kind gift from the Airola lab at Stony Brook University.