Primer

Part:BBa_K4361200

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-11)
Revision as of 07:17, 12 September 2022 by Larsvdb (Talk | contribs)


BlcR BTB primer R1

For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (BBa_K4361106) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer Rn" with n being the number of the set, and a number of forward primers, in this case F1.1 (BBa_K4361201) through F1.3 (BBa_K4361203). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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