Part:BBa_K4361200

BlcR BTB primer R1

For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (see <a href="https://parts.igem.org/Part:BBa_K4361106">BBa_K4361106</a>) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer Rn" with n being the number of the set, and a number of forward primers, in this case F1.1 (<a href="https://parts.igem.org/Part:BBa_K4361201">BBa_K4361201</a>) through F1.3 (<a href="https://parts.igem.org/Part:BBa_K4361203">BBa_K4361203</a>). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]