Part:BBa_K4390049
Linear Arsenic Biosensor
The Edinburgh-UHAS_Ghana team for 2022 designed a construct to detect arsenic in contaminated water. This construct is composed of a fluorescent RNA aptamer Spinach2 (BBa_K3773013) which is flanked by the F30 tRNA scaffolds (BBa_K3380101 and BBa_K3380102) which under a strong T7 RNA promoter (BBa_I712074), downstream of the promotor there is the binding site for the arsR repressor (BBa_J15101) as to allow transcriptional repression of the T7 promotor. This construct is designed to be cell-free and only requires transcription of the RNA aptamer to produce fluorescence. It should be noted that T7 RNA polymerase, chemical energy (ATP), NTPs and DFHBI or DFHO are also required in the cell-free reaction so that fluorescence is observed.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 52
Illegal SpeI site found at 190 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 52
Illegal SpeI site found at 190 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 52
Illegal SpeI site found at 190 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 52
Illegal SpeI site found at 190 - 1000COMPATIBLE WITH RFC[1000]
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