Regulatory

Part:BBa_K4235001:Design

Designed by: Maulik Masaliya, Lori Saxena, Stephanie Laderwager   Group: iGEM22_Stony_Brook   (2022-09-10)
Revision as of 22:18, 10 September 2022 by Maulik masaliya (Talk | contribs) (→‎References)

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Polyhedrin Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The polyhedrin promoter is a hyper-expression promoter that drives the expression of the polyhedrin gene found in baculoviruses. Due to its high expression power, the polyhedrin promoter may overwhelm a cell's protein production machinery when expressing multiple proteins, and deplete cellular resources along with a decrease in protein yield. Modifying the polyhedrin promoter to decrease its expression capacity can be used to optimize multiple protein expression yield ratios.


Source

This promoter is part of the vector pFastBac used for Bac-to-Bac expression system. We obtained the plasmid pFastBac as a generous donation from the Airola lab at Stony Brook University.

References

Putman, Z. (2020, November 15). Evaluating modified polyhedrin promoters for improved co-expression of KRAS4b. Maryland Shared Open Access Repository Home. Retrieved August 2, 2022, from https://mdsoar.org/handle/11603/20062