Device

Part:BBa_K4501005

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)
Revision as of 23:12, 8 September 2022 by Registry (Talk | contribs)

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MSCV 5' module

Encodes for the 5' LTR and the psi sequences, used on the 5' terminus of lentiviral transfer plasmids. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 780


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Categories
Parameters
None