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Part:BBa_K176035:Design

Designed by: Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-07-20)
Revision as of 06:30, 30 July 2009 by Horigen (Talk | contribs)

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TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1301
    Illegal BsaI.rc site found at 985


Design Notes

Input: AHL

Output: cell death

Design:

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and AHL, repressed by TetR and no aTc


Source

K176028 K176033

References