Cell

Part:BBa_K175003

Designed by: Calin Plesa   Group: iGEM09_TUDelft   (2009-07-07)
Revision as of 18:29, 29 July 2009 by Tim Weenink (Talk | contribs)

R751 plasmid with trbK knockout

Restriction site for the homing endonuclease I-SceI.

The cut will leave a 4bp overhang of ATAA.

5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'
3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5' Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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