Device

Part:BBa_K4175008

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-08-30)
Revision as of 05:08, 30 August 2022 by Registry (Talk | contribs)

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IL-6_scfv-Notch-Gal4KRAB

When the concentration of IL-6 is high, the binding of IL-6 with anti-IL-6 scFv (BBa_K4175000) will cause the Notch core domain (BBa_K4175001) undergo intracellular proteolytic cleavage. Consequently, Gal4-KRAB domain (BBa_K2446037) is released into the cytosol and subsequently, the nucleus. The DNA binding domain of Gal4 will bind to the UAS region, while the KRAB domain will inhibit the promoter next to the UAS region. In this way, the expression of the gene regulated by UAS is inhibited.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
    Illegal BglII site found at 1052
    Illegal BamHI site found at 76
    Illegal XhoI site found at 19
    Illegal XhoI site found at 1036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 2279
    Illegal NgoMIV site found at 2414
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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