Composite

Part:BBa_K4180007

Designed by: HuiChing Kuo   Group: iGEM22_KCIS_Xiugang_Taipei   (2022-08-29)
Revision as of 05:28, 29 August 2022 by Registry (Talk | contribs)

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pG1,10-snf1Δ381-633aa

This pG1,10-snf1Δ381-633aa is a composite biobrick. pGal1, 10-SPT5-Streptavidin Binding Protein(SBP) plasmid, containing 2u origin of replication (ORI) for yeast to start DNA replication, and ORI for bacteria DNA replication, was sponsored by Dr. Tien-Hsien Chang, at Genomics Research Center, Academia Sinica, in Taipei Taiwan. This plasmid can be transformed into bacteria and yeast, which was beneficial for our team to finish making biobricks in bacteria and perform the functional assay in yeast. Our team made 5 different basic parts (one promoter and 4 coding regions) and 4 different composite parts by inserting those 4 basic parts (BBa_K4180000, BBa_K4180002, BBa_K4180003, and BBa_K4180004) to replace the original SPT5 gene. pGal1,10-snf1Δ381-633, C-terminus truncated from amino acid381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR), losing the interaction with downstream of proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None