Composite

Part:BBa_K4180006

Designed by: HuiChing Kuo   Group: iGEM22_KCIS_Xiugang_Taipei   (2022-08-28)
Revision as of 23:58, 28 August 2022 by Registry (Talk | contribs)

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pGal1,10-snf1Δ2-306aa

This pGal1,10-snf1Δ2-306aa is a composite biobrick. pGal1, 10-SPT5-Streptavidin Binding Protein(SBP) plasmid, containing 2u origin of replication (ORI) for yeast to start DNA replication, and ORI for bacteria DNA replication, was sponsored by Dr. Tien-Hsien Chang, at Genomics Research Center, Academia Sinica, in Taipei Taiwan. This plasmid can be transformed into bacteria and yeast, which was beneficial for our team to finish making biobricks in bacteria and perform the functional assay in yeast. Our team made 5 different basic parts (one promoter and 4 coding regions) and 4 different composite parts by inserting those 4 basic parts (BBa_K4180000, BBa_K4180002, BBa_K4180003, and BBa_K4180004) to replace the original SPT5 gene. pGal1,10-snf1Δ2-306aa, N-terminus truncated from amino acid 2 to 306 deleting kinase domain of SNF1, where the phosphorylation on Thr210 activates the catalytic activity of SNF1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None