Protein_Domain

Part:BBa_K4175001:Design

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-08-27)
Revision as of 13:51, 28 August 2022 by JasLee24 (Talk | contribs)


Notch core


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 115
    Illegal PstI site found at 156
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 115
    Illegal PstI site found at 156
    Illegal PstI site found at 202
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 115
    Illegal PstI site found at 156
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 115
    Illegal PstI site found at 156
    Illegal PstI site found at 202
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been codon optimized.


Source

Mus musculus

References

Morsut, L., Roybal, K.T., Xiong, X., Gordley, R.M., Coyle, S.M., Thomson, M., Lim, W.A., 2016. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780–791. https://doi.org/10.1016/j.cell.2016.01.012