Coding

Part:BBa_K4271000

Designed by: Ethan Ho   Group: iGEM22_Wego_Taipei   (2022-08-27)
Revision as of 02:57, 28 August 2022 by JulieLin (Talk | contribs)


OPH (organophosphate hydrolase) + his-tag

Our experiment aims to resolve environmental eutrophication, which is caused by the abundance of organic phosphate and nitrogen in the water. OPH, a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. We use paraoxon as the substrate of OPH's degradation, after which it would be converted into diethyl phosphate (DEP) and p-nitrophenol (pNP). We use pNP as an indicator for spectrophotometric identification. his-tag is attached to the end of the OPH gene for the purification of the protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 193
    Illegal AgeI site found at 13
    Illegal AgeI site found at 298
    Illegal AgeI site found at 433
    Illegal AgeI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None