Coding

Part:BBa_K4348000:Design

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-08-22)
Revision as of 17:53, 22 August 2022 by Jonathancheng26 (Talk | contribs) (→‎Design Notes)


AKR1D1_his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was codon optimized for expression in E. coli One of the codons needed to be changed to remove an EcoRI site

Source

Genomic sequence from Eubacterium coprostanoligenes

References