Coding

Part:BBa_K4348001

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-08-22)
Revision as of 17:38, 22 August 2022 by Registry (Talk | contribs)

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ismA_his

An enzyme that converts cholesterol to 4-cholesten-3-one. It was discovered in Eubacterium coprostanoligenes by a team at the Broad Institute of MIT in 2021. This conversion is the first of the three-step pathway of converting cholesterol to coprostanol, a sterol that does not get absorbed by the gut. A 6x his-tag is attached to the N-terminal end, allowing for easy purification and analysis through nickel/cobalt columns and western blotting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None