Coding

Part:BBa_K4348000:Design

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-08-22)
Revision as of 17:32, 22 August 2022 by Registry (Talk | contribs)

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AKR1D1_his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An extra guanidine residue was added to the his tag to include an NcoI site. Amino acid sequence was codon optimized for expression in E. coli


Source

Genomic sequence from Eubacterium coprostanoligenes

References