Regulatory

Part:BBa_K4370006:Design

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-18)
Revision as of 16:54, 21 August 2022 by Sburymone (Talk | contribs) (→‎Source)


KasOP*_promoter_STREPTO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been designed for the expression of Streptomyces genes in the composite expression module BBa_BBa_K4370010.


Source

The sequence has been synthetized.

Chaoxian Bai , Yang Zhang , Xuejin Zhao, Yiling Hu, Sihai Xiang, Jin Miao, Chunbo Lou, Lixin Zhang “Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces”, Proc Natl Acad Sci U S A , 2015 Sep 29;112(39):12181-6. https://www.pnas.org/doi/10.1073/pnas.1511027112

Weishan Wang, Xiao Li, Juan Wang, Sihai Xiang, Xiaozhou Feng, Keqian Yang, “An engineered strong promoter for Streptomycetes”, Appl Environ Microbiol., 2013 Jul;79(14):4484-92. https://doi.org/10.1128/AEM.00985-13

References