RNA

Part:BBa_K4370012

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-18)
Revision as of 14:09, 18 August 2022 by Registry (Talk | contribs)

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sgRNA_gpm_STREPTO

This part encodes a single guide RNA targeting the gpm gene encoding a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (short name: phosphoglyceromutase) in Streptomyces . It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, and S. venezuelae ATCC 10712.

A decrease of gpm expression has been shown as required to allow the implementation of the Calcin cycle in E. coli (Antonovsky et al., Cell, 2016 - http://dx.doi.org/10.1016/j.cell.2016.05.064). This part could therefore be useful to implement a CO2 fixation module in Streptomyces.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None