RNA
Part:BBa_K4370012
Designed by: Stéphanie Bury-Moné Group: iGEM22_GO_Paris-Saclay (2022-08-18)
sgRNA_gpm_STREPTO
This part encodes a single guide RNA targeting the gpm gene encoding a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (short name: phosphoglyceromutase) in Streptomyces . It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, and S. venezuelae ATCC 10712.
A decrease of gpm expression has been shown as required to allow the implementation of the Calcin cycle in E. coli (Antonovsky et al., Cell, 2016 - http://dx.doi.org/10.1016/j.cell.2016.05.064). This part could therefore be useful to implement a CO2 fixation module in Streptomyces.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |