Regulatory

Part:BBa_K4159011:Design

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-08-12)
Revision as of 06:38, 12 August 2022 by Registry (Talk | contribs)

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pBAD and reversed P2 promoters


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For ordering the part, some changes might be needed in the beginning of the sequence as the beginning is very TA heavy. A few GC nucleotides will help the production to succeed when you order the part as a whole.


Source

The parts have been taken from the iGEM part registry.

References