Regulatory

Part:BBa_K4159011

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-08-12)
Revision as of 06:38, 12 August 2022 by Registry (Talk | contribs)

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pBAD and reversed P2 promoters

This part consists of two promoters pBAD, which is a continuous promoter, and of P2, which is an inducible promoter. We have placed the two promoters in two different direction, P2 being reversed and pBAD in the left to right direction. This way we try to avoid false positive results with the downstream genes, incase either promoter is a little "leaky". Between the promoter we have a spacer sequence and after both promoters we have placed the B0034 RBS sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None