![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4150004
Designed by: WEI CHIEH CHIN Group: iGEM22_Mingdao (2022-08-11)
His-OVA
The chicken ovalbumin sequence obtained from KEGG database (Gallus gallus) was synthesized by Integrated DNA Technologies, Inc. and connected with a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 373
[edit]
Categories
Parameters
//awards/basic_part/nominee
None |