Coding

Part:BBa_K4390025

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)


Si-tag :: Gly-Ser Linker

This part attaches a Si-tag separated by a Gly-Ser linker to a protein. This part was designed as a Type NO part for JUMP assembly. Missing TTCG at the end to complete the sequence, as this is regenerated by the O part 3' fusion site in JUMP assembly. Add TTCG at the end to use as a normal part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 14
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None