Composite

Part:BBa_K4390020:Design

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 13:03, 7 August 2022 by Registry (Talk | contribs)

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Danio rerio MT expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This compound (level 1) part was assembled using JUMP, a type IIS assembly method, so all basic (level 0) parts had to have the correct JUMP fusion sites flanking, as well as BsaI sites for level 1 assembly and BsmBI sites for level 0 assembly.



Source

Yan, C.H.M., Chan, K.M., 2004. Cloning of zebrafish metallothionein gene and characterization of its gene promoter region in HepG2 cell line. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. https://doi.org/10.1016/j.bbaexp.2004.04.004


References