Coding

Part:BBa_K4390016:Design

Designed by: Maarten van den Ancker, Serafina Soehianto   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 12:45, 7 August 2022 by Maarten vandenancker (Talk | contribs) (→‎Design Notes)


6xHis :: TEV protease cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be used in JUMP assembly as a N part, so the last four nucleotides in the cleavage site have been removed, as they will be regenerated by the N part 3' fusion site (AGCC), creating a scarless final assembly. To use this sequence in non-JUMP assembly, add AGCC to the end of the sequence.

Source

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References