Coding

Part:BBa_K4390014:Design

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 12:18, 7 August 2022 by Registry (Talk | contribs)

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Saccharomyces cerevisiae Metallothionein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
    Illegal PstI site found at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 93
    Illegal PstI site found at 171
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
    Illegal PstI site found at 171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal PstI site found at 171
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimised for expression in E. coli K12. The native stop codon has been removed and replaced with a double stop codon.



Source

Sayers, Z., Brouillon, P., Svergun, D.I., Zielenkiewicz, P., Koch, M.H.J., 1999. Biochemical and structural characterization of recombinant copper-metallothionein from Saccharomyces cerevisiae. Eur J Biochem. https://doi.org/10.1046/j.1432-1327.1999.00451.x

References