Coding

Part:BBa_K4390013:Design

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 12:14, 7 August 2022 by Registry (Talk | contribs)

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Pseudomonas fluorescens Metallothionein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 214


Design Notes

This part has been codon optimised for expression in E. coli K12. The native stop codon has been removed and replaced with a double stop codon.



Source

Habjanič, J., Zerbe, O., Freisinger, E., 2018. A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc. Metallomics. https://doi.org/10.1039/c8mt00193f

References