Coding
Part:BBa_K4390011:Design
Designed by: Maarten van den Ancker Group: iGEM22_Edinburgh-UHAS_Ghana (2022-08-07)
Callinectes sapidus Metallothionein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been codon optimised for expression in E. coli K12. The native stop codon has been removed and replaced with a double stop codon.
Source
Brouwer, M., Enghild, J., Hoexum-Brouwer, T., Thogersen, I., Truncali, A., 1995. Primary structure and tissue-specific expression of blue crab (Callinectes sapidus) metallothionein isoforms. Biochemical Journal. https://doi.org/10.1042/bj3110617