Coding

Part:BBa_K4475000:Design

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-07-17)
Revision as of 23:21, 31 July 2022 by Andrew Taylor (Talk | contribs) (→‎Design Notes)


CTB1 Gene from Cercospora beticola


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3532
    Illegal BamHI site found at 3441
    Illegal BamHI site found at 5614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6121
    Illegal AgeI site found at 4195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was determined from an mRNA sample of Cercospora Beticola (listed under references). The sequence was also codon optimized for yeast, silently mutated to avoid restriction enzyme cut sites and tagged with an N-terminus HA tag.

Source

Genome of cercospora beticola

References

https://www.ncbi.nlm.nih.gov/nuccore/1331157726