Coding

Part:BBa_K4268000:Design

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski   Group: iGEM22_SUNY_Oneonta   (2022-07-15)
Revision as of 21:09, 26 July 2022 by Jazmine124 (Talk | contribs) (→‎Source)


S-TIP37 Head-Tail Connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The sequence was edited to ensure that it is RCF10 and RCF1000 compatible.
  • The sequence contains SapI sites at the ends that are used in Level 0 Golden Gate Assembly.

Source

The source of this sequence is from the S-TIP37 genome (GenBanK: NC_048026.1)

References

The S-TIP37 genome was found on GenomeNet