Part:BBa_K4347011:Design

Bst fusion with Sac7e and point mutations for enhanced thermal stability codon optimized for E.coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 5
  Illegal XhoI site found at 209
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1015
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Bst Mutagenesis

For more information and a complete overview on how the mutated polymerase was designed please view: https://parts.igem.org/Part:BBa_K4347007:Design

Research that lead to choosing sac7e as ssb protien. Alpha fold analysis Yasara analysis

Considerations

Point mutations were made for thermal stability to account for fluctuations in the portable heating device, as temperature fluctuations typically oscillate about the desired set point temperature when using electronic circuits.

Section can be summary from https://parts.igem.org/Part:BBa_K4347007# and https://parts.igem.org/Part:BBa_K4347010.

Source

• Sac7e: https://parts.igem.org/Part:BBa_K4347006
• (GGGGS)₄ linker: https://parts.igem.org/Part:BBa_K3117004
• Bst: https://parts.igem.org/Part:BBa_K4347010