Plasmid_Backbone

Part:BBa_K4516006

Designed by: Kong Yangyang   Group: iGEM22_Jiangsu_United   (2022-07-19)
Revision as of 09:27, 19 July 2022 by Kimmy2020 (Talk | contribs)

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pcDNA3.1-backbone

pcDNA3.1-backbone

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 312
    Illegal PstI site found at 1647
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 292
    Illegal PstI site found at 1647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 12
    Illegal XhoI site found at 318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 312
    Illegal PstI site found at 1647
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 312
    Illegal PstI site found at 1647
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 2098
    Illegal NgoMIV site found at 2381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 279
    Illegal BsaI.rc site found at 3906
    Illegal SapI site found at 2826
    Illegal SapI.rc site found at 1947
    Illegal SapI.rc site found at 2157


[edit]
Categories
Parameters
None