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Part:BBa_K4475004:Design

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-07-18)
Revision as of 05:40, 19 July 2022 by Andrew Taylor (Talk | contribs)


CYC1 Terminator Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native coding sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.


Source

Coding sequence from cercospora beticola.

References