Coding
Bst Mut

Part:BBa_K4347007

Designed by: Victor Di Donato, Nicoletta de Maat   Group: iGEM22_Queens_Canada   (2022-07-07)
Revision as of 15:03, 8 July 2022 by Victor5688 (Talk | contribs)

Bst with point mutations for enhanced thermal stability codon optimized for E.coli

small introduction about bst here... eg, what is it? what organism does it come from? Improvement from https://parts.igem.org/Part:BBa_K3790000 bst part from iGEM Fudon.

Usage and Biology

How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc...

Enhanced Thermal stability

Talk about the point mutations. Pictures of lab experiments


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[edit]
Categories
Parameters
None