Coding
Bst Mut

Part:BBa_K4347007

Designed by: Victor Di Donato, Nicoletta de Maat   Group: iGEM22_Queens_Canada   (2022-07-07)
Revision as of 14:53, 8 July 2022 by Victor5688 (Talk | contribs)

Bst with point mutations for enhanced thermal stability codon optimized for E.coli

small introduction about bst here... eg, what is it? what organism does it come from?

Usage and Biology

How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc...

Enhanced Thermal stability

Talk about the point mutations. Pictures of lab experiments


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


References

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Categories
Parameters
None