Device

Part:BBa_K176030:Design

Designed by: Chao Li, Danqian Liu, Hao Jiang   Group: iGEM09_USTC   (2009-07-20)
Revision as of 14:00, 20 July 2009 by Horigen (Talk | contribs) (→‎Design Notes)

Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 855
    Illegal NheI site found at 878
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1833
    Illegal BsaI.rc site found at 2577


Design Notes

Input1: PoPS

Input2: AHL

Output: GFP

Design:

Input1 PoPS generate TetR-LVA

pCon 0.70(J23101) generate LuxR

pLux/Tet(K176000) generate GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc

Source

K176006 K176026

References