Plasmid_Backbone
Part:BBa_J428342
Designed by: Marcos Valenzuela-Ortega Group: 2021 Engineering Committee (2022-06-15)
pJUMP29-1B KanR Type IIS Level 1 vector for B modules
OriV 9 (pBBR322/ROP; medium copy number)
Sequence and Features Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2948
Illegal suffix found at 16 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2948
Illegal NheI site found at 2
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NotI site found at 24
Illegal NotI site found at 2954 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2948 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2948
Illegal suffix found at 17 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2948
Plasmid lacks a suffix.
Illegal XbaI site found at 2963
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NgoMIV site found at 1390 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2188
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
origin | pBBR322 |
resistance | kanamycin |