Part:BBa_J428341

pJUMP29-1A KanR Type IIS Level 1 vector for A modules

OriV 9 (pBBR322/ROP; medium copy number)

Sequence and Features

Please view and default to the original genbank files from the Supplementary Data https://doi.org/10.1093/synbio/ysab003

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 2948
  Illegal suffix found at 16
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2948
  Illegal SpeI site found at 17
  Illegal PstI site found at 31
  Illegal NotI site found at 24
  Illegal NotI site found at 2954
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2948
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2948
  Illegal suffix found at 17
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2948
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2963
  Illegal SpeI site found at 17
  Illegal PstI site found at 31
  Illegal NgoMIV site found at 1390
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 2188