Part:BBa_F2620

3OC₆HSL -> PoPS Receiver

This device senses the 3OC₆HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.

Usage and Biology

<img src="Include File F2620Data"

The transfer function indicates a switch point of approximately 2nM which was constant in repeated experiments (data not shown).

The latency shows that fluorescent proteins appear within moments of the addition of AHL to the media. This suggests that PoPS output adjusts to a change in input very rapidly. This is expected given the current model of device operation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1004
  

Functional Parameters

control	R0040
device_type	Receiver
family	-NA-
latency	Seconds
proteins	LuxR, TetR
signalling_molecule	3OC6HSL
switch_point	2nM