Signalling

Part:BBa_K176026:Design

Designed by: Danqian Liu, Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-07-16)
Revision as of 13:22, 20 July 2009 by Horigen (Talk | contribs) (→‎Design Notes)

TetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1729


Design Notes

Input: AHL

Output: GFP

Design:

pCon 0.70(J23101) generate LuxR

pLux/Tet(K176000) generate GFP, activated by LuxR and AHL, repressed by TetR and no aTc

Source

K176025 K176002

References