Coding

Part:BBa_K4421010

Designed by: Shuyang Zhu   Group: iGEM22_NMU_China   (2022-06-21)
Revision as of 13:08, 21 June 2022 by Juejue (Talk | contribs)


2A sequence

The 2A peptide sequence, serving as a self-cleaving sequence， was intercalated between iCASP9 and the GFP tag and it is derived from FMDV (foot-and-mouth disease virus) to allow transcription and expression of one single mRNA molecule.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None