Plasmid_Backbone

Part:BBa_J428363

Designed by: Marcos Valenzuela-Ortega   Group: 2021 Engineering Committee   (2022-06-15)
Revision as of 18:15, 15 June 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pJUMP49-2C SpecR Type IIS Level 2 vector for C modules

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2991
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2991
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2997
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2991
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2991
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2991
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3006
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1039
    Illegal NgoMIV site found at 1441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3013
    Illegal BsaI.rc site found at 10
    Illegal SapI.rc site found at 2239


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/jump/level2
//plasmidbackbone/assembly/typeiis
Parameters
resistanceSpectinomycin